DNA vaccine and Application of DNA vaccine in “Fisheries”

DNA vaccine and Application of DNA vaccine in “Fisheries”Photo Albumby Activated UserPhoto Albumby Activated UserDNA vaccine is DNA sequence used as a vaccineThis DNA sequence code for antigenic protein of pathogen As this DNA inserted into cells it is translated to form antigenic protein. As this protein is foreign to cells, so immune response raised against this protein.In this way, DNA vaccine provide immunity against that pathogen.Photo Albumby Activated UserProduct DNA vaccineDNAVaccinePathogenIHN is an RNA stranded virus. The genome codes for 6 different proteins are given. Five are structural proteins and one is a non structural protein. Must know which gene codes for the protein that is involved in the immunetic response. It is the glyco protein spike on the outside of the virus, denoted by the G protein, that is the immunetic protein of the virus.Insect G-gene into DNA plasmid form Recombinant DNAInject DNA vaccine into FishG-gene construct shows good surface expression, which is believed to be a key feature to the effectiveness of the vaccine. You also see a very heavy monocyticcell infiltrate, most likely white blood cells.Queries ? Tom Christian Tonheim*, Jarl Bøgwald, Roy Ambli Dalmo **. What happens to the DNA vaccine in fish? 19 March 2008 	Trần Tuấn Anh	Vũ Mạnh Công	Nông Văn Cương	Vi Đại Lâm 	Nguyễn Đức Thành	Hoàng Đình ViệtMany thanks to all of you!